Journal
/
/
单个肌纤维和成人骨骼肌卫星细胞的分离和培养
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
DOI:

11:57 min

March 22, 2013

, ,

Chapters

  • 00:05Title
  • 01:44Preparations
  • 03:33Muscle Dissection and Digestion
  • 07:00Single Myofiber Dissociation and Culture
  • 09:35Ex vivo Expression of Pax7 and Myf5 in Myofibers
  • 11:02Conclusion

Summary

Automatic Translation

肌纤维的分离和培养是黄金标准<em在体外</em系统研究通过平静,激活和分化的过渡卫星细胞。重要的是,单个肌纤维的文化系统保留的肌纤维/干细胞协会,这是一个必不可少的组成部分的肌肉干细胞小生境。

Related Videos

Read Article