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单个肌纤维和成人骨骼肌卫星细胞的分离和培养
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
Please note that all translations are automatically generated.
Click here for the English version.
单个肌纤维和成人骨骼肌卫星细胞的分离和培养
DOI:
10.3791/50074-v
•
11:57 min
•
March 22, 2013
•
Alessandra Pasut
2
,
Andrew E. Jones
2
,
Michael A. Rudnicki
2
1
Sprott Center for Stem Cell Research
,
Ottawa Hospital Research Institute
,
2
Department of Cellular and Molecular Medicine
,
University of Ottawa
Chapters
00:05
Title
01:44
Preparations
03:33
Muscle Dissection and Digestion
07:00
Single Myofiber Dissociation and Culture
09:35
Ex vivo Expression of Pax7 and Myf5 in Myofibers
11:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
肌纤维的分离和培养是黄金标准<em在体外</em系统研究通过平静，激活和分化的过渡卫星细胞。重要的是，单个肌纤维的文化系统保留的肌纤维/干细胞协会，这是一个必不可少的组成部分的肌肉干细胞小生境。
Tags
Myofiber Isolation
Satellite Cells
Muscle Regeneration
Bischoff Protocol
Rosenblatt Protocol
Muscle Stem Cells
Single Myofiber Culture
Quiescence
Activation
Proliferation
Differentiation
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