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使用高通量形状的RNA二级结构预测
JoVE Journal
Biology
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JoVE Journal Biology
RNA Secondary Structure Prediction Using High-throughput SHAPE
DOI:

13:42 min

May 31, 2013

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Chapters

  • 00:05Title
  • 01:40RNA Folding
  • 03:09Chemical Modification of the RNA
  • 04:33Reverse Transcription
  • 07:08Preparation of Sequencing Ladder and Capillary Electrophoresis
  • 09:55ShapeFinder Software
  • 11:15Data Normalization
  • 11:51Results: RNA Structure Determination using RNA Structure Software
  • 12:53Conclusion

Summary

Automatic Translation

的高通量选择性2'羟基的酰化反应分析的引物延伸(SHAPE)利用了一种新的化学探测技术,反转录,毛细管电泳确定结构的RNA二级结构预测软件从几百到几千个核苷酸,单核苷酸分辨率。

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