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使用自制双辉光萤光素酶检测的高通量筛选功能
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High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
Please note that all translations are automatically generated.
Click here for the English version.
使用自制双辉光萤光素酶检测的高通量筛选功能
DOI:
10.3791/50282-v
•
12:55 min
•
June 01, 2014
•
Jessica M. Baker
,
Frederick M. Boyce
1
Department of Neurology
,
Massachusetts General Hospital
Chapters
00:05
Title
01:34
Preparing 293T Cells and Reporter Plasmids for Screening
03:37
Transfections
06:48
Dual-Luciferase Assays
09:20
Results: Identification of Novel Transcription Regulators of Alpha-synuclein (SNCA)
12:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们目前确定采用高通量机器人转染和一个自制的双发光荧光素酶检测转录调控因子的快速和廉价的检查方法。该协议迅速产生直接并排端功能数据数以千计的基因,很容易修改针对任何感兴趣的基因。
Tags
High-throughput Screening
Functional Screening
Dual-glow Luciferase Assay
Alpha-synuclein
Parkinson’s Disease
293T Cells
ORF Expression Library
Firefly Luciferase
Renilla Luciferase
Automated Transfection
Lentiviral Library Production
PTC124
EDTA
Pyrophosphate
Gene Regulators
Article
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