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使用自制双辉光萤光素酶检测的高通量筛选功能
JoVE Journal
Biology
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JoVE Journal Biology
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
DOI:

12:55 min

June 01, 2014

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Chapters

  • 00:05Title
  • 01:34Preparing 293T Cells and Reporter Plasmids for Screening
  • 03:37Transfections
  • 06:48Dual-Luciferase Assays
  • 09:20Results: Identification of Novel Transcription Regulators of Alpha-synuclein (SNCA)
  • 12:28Conclusion

Summary

Automatic Translation

我们目前确定采用高通量机器人转染和一个自制的双发光荧光素酶检测转录调控因子的快速和廉价的检查方法。该协议迅速产生直接并排端功能数据数以千计的基因,很容易修改针对任何感兴趣的基因。

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