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12:55 min
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June 01, 2014
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Summary
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Nous présentons une méthode de dépistage rapide et peu coûteuse pour identifier des régulateurs de transcription à l'aide de haut-débit transfections robotiques et un dosage de la luciférase double lueur maison. Ce protocole génère rapidement des données fonctionnelles directes côté-à-côte pour des milliers de gènes et est facilement modifiable pour cibler tout gène d'intérêt.
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Cite this Article
Baker, J. M., Boyce, F. M. High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay. J. Vis. Exp. (88), e50282, doi:10.3791/50282 (2014).
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