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Lev Imaging Assay for at vurdere de roller Ca<sup> 2 +</sup> Og sphingomyelinase i reparation af poredannende Toxin Wounds
JoVE Journal
Biology
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JoVE Journal Biology
Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds
DOI:

18:25 min

August 25, 2013

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Chapters

  • 00:05Title
  • 01:41Transcriptional Silencing of ASM
  • 04:18Preparation of Reagents for Live Microscopy
  • 05:29Live Cell Imaging of FM1-43 Influx into SLO Treated and Untreated Cells
  • 10:21Quantification of FM1-43 Influx into Cells
  • 14:03Results: Ca2+ and Sphingomyelinase are Involved in Plasma Membrane Repair
  • 17:34Conclusion

Summary

Automatic Translation

Levende billeddannelse af celler udsat for den lipofile farvestof FM1-43 muliggør en præcis bestemmelse af kinetikken, som poredannende toksiner er fjernet fra plasmamembranen. Det er et følsomt assay, der kan bruges til at vurdere kravene til Ca 2 +, sphingomyelinase og andre faktorer på plasma membran reparation.

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