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Egenrapportering Stillaser for 3-dimensjonal Cell Culture
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Self-reporting Scaffolds for 3-Dimensional Cell Culture
DOI:

14:49 min

November 07, 2013

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Chapters

  • 00:05Title
  • 01:36Preparation and Analysis of pH Responsive Sol-gel Nanosensors
  • 04:26Preparation and Analysis of PLGA Scaffolds
  • 06:57Nanosensor Location within Mammalian Cells
  • 08:36Nanosensor Delivery to Cells Cultured upon Regular PLGA Scaffolds
  • 10:33Nanosensor Location within Mammalian Cells
  • 11:57Results: Incorporation of Nanosensors into Scaffolds and Cells
  • 14:20Conclusion

Summary

Automatic Translation

Biokompatible pH responsive sol-gel nanosensorer kan bli innlemmet i poly (melkesyre-co-glykolsyre) (PLGA) elektrospunnede stillaser. De produserte egenrapportering stillaser kan brukes for in situ overvåking av microenviron forhold mens dyrking celler på stillaset. Dette er fordelaktig da den 3D cellekonstruksjon kan overvåkes i sanntid, uten å forstyrre forsøket.

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