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Egenrapportering Stillaser for 3-dimensjonal Cell Culture
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Bioengineering
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JoVE Journal Bioengineering
Self-reporting Scaffolds for 3-Dimensional Cell Culture

Egenrapportering Stillaser for 3-dimensjonal Cell Culture

DOI:
10.3791/50608-v

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14:49 min

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November 07, 2013

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, , ,
1School of Molecular Medical Sciences,University of Nottingham, 2Division of Drug Delivery and Tissue Engineering,University of Nottingham, 3Laboratory of Biophysics and Surface Analysis,University of Nottingham

Chapters

  • 00:05Title
  • 01:36Preparation and Analysis of pH Responsive Sol-gel Nanosensors
  • 04:26Preparation and Analysis of PLGA Scaffolds
  • 06:57Nanosensor Location within Mammalian Cells
  • 08:36Nanosensor Delivery to Cells Cultured upon Regular PLGA Scaffolds
  • 10:33Nanosensor Location within Mammalian Cells
  • 11:57Results: Incorporation of Nanosensors into Scaffolds and Cells
  • 14:20Conclusion

Summary

Automatic Translation

Automatic Translation

Biokompatible pH responsive sol-gel nanosensorer kan bli innlemmet i poly (melkesyre-co-glykolsyre) (PLGA) elektrospunnede stillaser. De produserte egenrapportering stillaser kan brukes for in situ overvåking av microenviron forhold mens dyrking celler på stillaset. Dette er fordelaktig da den 3D cellekonstruksjon kan overvåkes i sanntid, uten å forstyrre forsøket.

Tags

3D Cell CultureScaffoldsPLGAPH-responsive NanosensorsElectrospun ScaffoldsMicroenvironmentCell-cell InteractionsIntracellular PH

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