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10:06 min
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October 19, 2013
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Summary
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Il pesce zebra, un piccolo pesce tropicale, è diventata un modello importante per studiare la funzione del gene durante lo sviluppo dei vertebrati e la malattia. L'espressione temporale e spaziale dei geni bersaglio può essere determinata<em> In situ</em> Ibridazione. Nostra migliore protocollo permette l'individuazione di bassi trascritti abbondanti con basso segnale sfondo non specifico.
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Cite this Article
Chitramuthu, B. P., Bennett, H. P. J. High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function. J. Vis. Exp. (80), e50644, doi:10.3791/50644 (2013).
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