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从人类胸腺髓样树突状细胞和上皮细胞的分离
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
DOI:

09:07 min

September 19, 2013

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Chapters

  • 00:05Title
  • 01:11Preparation of the Tissue
  • 02:45Typical Enzymatic Tissue Digestion to Obtain a Single Cell Suspension
  • 04:35Enrichment of Low Density Fraction (LDF) of Cells Using Percoll Density Separation
  • 06:00Results: Representative Myeloid DC and TEC Populations Isolated from the Human Thymus
  • 08:06Conclusion

Summary

Automatic Translation

这个协议的细节的方法来分离抗原通过不同的步骤的组织随后在单细胞悬浮液的密度离心和感兴趣的细胞群的最后磁和/或FACS分选的酶消化从人胸腺呈递细胞。

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