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Journal
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Immunology and Infection
/
从人类胸腺髓样树突状细胞和上皮细胞的分离
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Immunology and Infection
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Immunology and Infection
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
Please note that all translations are automatically generated.
Click here for the English version.
从人类胸腺髓样树突状细胞和上皮细胞的分离
DOI:
10.3791/50951-v
•
09:07 min
•
September 19, 2013
•
Christina Stoeckle
2
,
Ioanna A. Rota
,
Eva Tolosa
,
Christoph Haller
,
Arthur Melms
,
Eleni Adamopoulou
1
Department of General Neurology
,
Hertie Institute for Clinical Brain Research
,
2
Institute of Pharmacology
,
University of Bern
,
3
Department of Immunology
,
University Medical Center Hamburg-Eppendorf
,
4
Department of Thoracic and Cardiovascular Surgery
,
University Clinic Tuebingen
,
5
Department of Neurology
,
University Hospital Erlangen
Chapters
00:05
Title
01:11
Preparation of the Tissue
02:45
Typical Enzymatic Tissue Digestion to Obtain a Single Cell Suspension
04:35
Enrichment of Low Density Fraction (LDF) of Cells Using Percoll Density Separation
06:00
Results: Representative Myeloid DC and TEC Populations Isolated from the Human Thymus
08:06
Conclusion
Summary
Automatic Translation
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Automatic Translation
这个协议的细节的方法来分离抗原通过不同的步骤的组织随后在单细胞悬浮液的密度离心和感兴趣的细胞群的最后磁和/或FACS分选的酶消化从人胸腺呈递细胞。
Tags
Myeloid Dendritic Cells
Epithelial Cells
Thymus
Antigen Presenting Cells
Cell Isolation
Percoll Density Centrifugation
Magnetic Cell Sorting
FACS
Cell Characterization
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