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ヒト胸腺からの骨髄樹状細胞と上皮細胞の単離
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
DOI:

09:07 min

September 19, 2013

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Chapters

  • 00:05Title
  • 01:11Preparation of the Tissue
  • 02:45Typical Enzymatic Tissue Digestion to Obtain a Single Cell Suspension
  • 04:35Enrichment of Low Density Fraction (LDF) of Cells Using Percoll Density Separation
  • 06:00Results: Representative Myeloid DC and TEC Populations Isolated from the Human Thymus
  • 08:06Conclusion

Summary

Automatic Translation

このプロトコルは、濃度の単一細胞懸濁液を遠心分離し、最後に、磁気および/または目的の細胞集団のFACS選別に続いて、組織の酵素消化の異なるステップを介して、ヒト胸腺から抗原提示細胞を単離する方法を詳述する。

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