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En cGMP-gældende Expansion Metode til aggregater af humane neurale Stem og stamceller arvet fra pluripotente stamceller eller fostrets hjerne Tissue
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Chapters
- 00:05Title
- 01:36hNPC Observations
- 02:13Chopper Setup
- 03:22Pre-chop Procedure
- 05:58Chop Procedure
- 07:08Post-chop Procedure
- 08:11Representative Results of hNPCs Aggregates Using a cGMP-applicable Expansion Method
- 09:04Conclusion
Denne protokol beskriver en ny mekanisk sønderdeling, der tillader udvidelse af sfæriske neurale stamceller og stamcelle aggregater uden dissociation til en enkelt cellesuspension. Fastholdelse af celle / celle kontakt giver mulighed for hurtig og stabil vækst i over 40 passager.
