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在乳腺癌细胞雌激素调节小分子RNA的剖析
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
DOI:

16:24 min

February 21, 2014

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Chapters

  • 00:05Title
  • 01:31Cell Preparation and Treatment
  • 04:19RNA Extraction
  • 06:40miRNA Profiling Analysis
  • 08:17Confirmation of miRNA Regulations using Separate qPCR Analysis
  • 10:49Results: Estrogen Regulates miRNA in Breast Cancer Cells
  • 15:36Conclusion

Summary

Automatic Translation

分子通过雌激素和小分子RNA的信号是在乳腺癌的发展和增长的关键。雌激素激活的雌激素受体,它是转录因子。许多转录因子能调节微RNA的表达,而雌激素调节的微小RNA可以使用不同的大规模的技术来成型。

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