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在乳腺癌细胞雌激素调节小分子RNA的剖析
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
Please note that all translations are automatically generated.
Click here for the English version.
在乳腺癌细胞雌激素调节小分子RNA的剖析
DOI:
10.3791/51285-v
•
16:24 min
•
February 21, 2014
•
Anne Katchy
,
Cecilia Williams
1
Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry
,
University of Houston
Chapters
00:05
Title
01:31
Cell Preparation and Treatment
04:19
RNA Extraction
06:40
miRNA Profiling Analysis
08:17
Confirmation of miRNA Regulations using Separate qPCR Analysis
10:49
Results: Estrogen Regulates miRNA in Breast Cancer Cells
15:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
分子通过雌激素和小分子RNA的信号是在乳腺癌的发展和增长的关键。雌激素激活的雌激素受体,它是转录因子。许多转录因子能调节微RNA的表达,而雌激素调节的微小RNA可以使用不同的大规模的技术来成型。
Tags
Estrogen
Estrogen Receptor
Breast Cancer
MiRNA
Microarray
QPCR
Gene Expression
Post-transcriptional Regulation
Oncogene
Tumor Suppressor
Biomarker
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