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Chapters
- 00:05Title
- 01:19Protein Purification and Alkylation of Free Sulfhydryl Groups
- 02:59Protein Digestion, Reversed Phase Peptide Extraction, and Reductive Dimethylation Labeling
- 06:02Basic pH Reversed Phase Chromatography and STop and Go Extraction
- 08:17Microcapillary LC-MS/MS and Peptide Quantification
- 09:58Results: Accuracy, Precision, and Reproducibility of ReDi Labeling
- 11:24Conclusion
Stabil isotop mærkning af peptider ved reduktiv dimethylation (redi mærkning) er en hurtig, billig strategi for nøjagtige massespektrometri-baserede kvantitative proteomics. Her demonstrerer vi en robust metode til forberedelse og analyse af proteinblandinger hjælp Redi tilgang, der kan anvendes til næsten enhver prøvetype.
Tags
Quantitative ProteomicsReductive DimethylationStable Isotope LabelingMass SpectrometryProtein Expression DifferencesRegular/formaldehyde LabelingDeuterated LabelingSodium CyanoborohydrideMethyl TagsLC-MS/MS AnalysisIon Chromatogram Peak AreasSample PreparationReversed-phase Solid Phase ExtractionBasic PH Reversed-phase ChromatographyStageTip Peptide PurificationAdvantages Of ReDi LabelingLimitations Of ReDi LabelingNovel Applications Of ReDi Labeling
