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Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
JoVE Journal
Biology
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JoVE Journal Biology
Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
DOI:

09:41 min

June 17, 2014

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Chapters

  • 00:05Title
  • 01:41Depletion from Xenopus Extract
  • 02:57Optimizing Xenopus Extract for β-catenin Degradation
  • 04:10Radiolabeled β-catenin Degradation Assay in Xenopus Egg Extract
  • 05:47β-catenin-luciferase Degradation Assay in Xenopus Egg Extract
  • 07:24Results: Analysis of β-catenin Degradation
  • 09:03Conclusion

Summary

Automatic Translation

A method is described for analyzing protein degradation using radiolabeled and luciferase-fusion proteins in Xenopus egg extract and its adaptation for high-throughput screening for small molecule modulators of protein degradation.

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