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Journal / Biology / Reconstituição de β-catenina degradação na
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Reconstituição de β-catenina degradação na
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Reconstituição de β-catenina degradação na

Article DOI: 10.3791/51425 • 09:41 min • June 17th, 2014
June 17th, 2014 •
*1, *1, 2, 1,3
1Department of Cell and Developmental Biology and Program in Developmental Biology, Vanderbilt University Medical Center, 2Division of Gastroenterology, Hepatology & Nutrition and Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 3Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine
* These authors contributed equally

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Um método é descrito para a análise de degradação de proteínas utilizando radiolabeled e proteínas luciferase de fusão em Xenopus extrato do ovo e sua adaptação para high-throughput screening para modulares de degradação de proteínas.

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Molecular Biology Edição 88, a degradação de proteínas radioactivo luciferase autoradiografia high-throughput screening
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