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重组β-catenin的降解<em>爪蟾</em>卵提取物
JoVE Journal
Biology
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JoVE Journal
Biology
Reconstitution Of β-catenin Degradation In
Xenopus
Egg Extract
Please note that all translations are automatically generated.
Click here for the English version.
重组β-catenin的降解<em>爪蟾</em>卵提取物
DOI:
10.3791/51425-v
•
09:41 min
•
June 17, 2014
•
Tony W. Chen*
1
,
Matthew R. Broadus*
1
,
Stacey S. Huppert
,
Ethan Lee
3
1
Department of Cell and Developmental Biology and Program in Developmental Biology
,
Vanderbilt University Medical Center
,
2
Division of Gastroenterology, Hepatology & Nutrition and Division of Developmental Biology
,
Cincinnati Children's Hospital Medical Center
,
3
Vanderbilt Ingram Cancer Center
,
Vanderbilt University School of Medicine
Chapters
00:05
Title
01:41
Depletion from
Xenopus
Extract
02:57
Optimizing
Xenopus
Extract for β-catenin Degradation
04:10
Radiolabeled β-catenin Degradation Assay in
Xenopus
Egg Extract
05:47
β-catenin-luciferase Degradation Assay in
Xenopus
Egg Extract
07:24
Results: Analysis of β-catenin Degradation
09:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
的方法被描述为使用放射性标记和荧光素酶融合蛋白在
非洲蟾蜍
卵提取物和其适应高通量筛选用于蛋白质降解的小分子调节剂进行分析的蛋白质降解。
Tags
Xenopus Egg Extract
β-catenin Degradation
Protein Turnover
Cell Cycle
Signal Transduction
Wnt Pathway
[35S]-radiolabeled Proteins
Firefly Luciferase-tagged Fusion Proteins
High-throughput Screening
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