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研究蛋白质 - 蛋白质相互作用活细胞使用生物发光共振能量转移
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JoVE Journal
Biology
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
Please note that all translations are automatically generated.
Click here for the English version.
研究蛋白质 - 蛋白质相互作用活细胞使用生物发光共振能量转移
DOI:
10.3791/51438-v
•
11:46 min
•
May 26, 2014
•
Pelagia Deriziotis*
1
,
Sarah A. Graham*
1
,
Sara B. Estruch
,
Simon E. Fisher
2
1
Language and Genetics Department
,
Max Planck Institute for Psycholinguistics
,
2
Donders Institute for Brain, Cognition and Behaviour
Chapters
00:05
Title
01:36
Preparation of DNA Mixes
04:25
Transfection
05:59
Measurement of BRET Signal
07:14
Results: Assessing FOXP2 Protein-protein Interactions Using BRET
11:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
蛋白质之间的相互作用是十分重要的所有细胞过程。使用生物发光共振能量转移,一对蛋白质之间的相互作用可以在活细胞中,并实时监测。此外,潜在的致病突变的效果进行评估。
Tags
Bioluminescence Resonance Energy Transfer (BRET)
Protein-protein Interactions
Live Cells
Renilla Reniformis Luciferase
Yellow Fluorescent Protein (YFP)
Fusion Proteins
Mammalian Cells
Yeast Two-hybrid
Mass Spectrometry Proteomics
Post-translational Modifications
Mutations
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