Journal
/
/
研究蛋白质 - 蛋白质相互作用活细胞使用生物发光共振能量转移
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
DOI:

11:46 min

May 26, 2014

, , ,

Chapters

  • 00:05Title
  • 01:36Preparation of DNA Mixes
  • 04:25Transfection
  • 05:59Measurement of BRET Signal
  • 07:14Results: Assessing FOXP2 Protein-protein Interactions Using BRET
  • 11:09Conclusion

Summary

Automatic Translation

蛋白质之间的相互作用是十分重要的所有细胞过程。使用生物发光共振能量转移,一对蛋白质之间的相互作用可以在活细胞中,并实时监测。此外,潜在的致病突变的效果进行评估。

Related Videos

Read Article