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Feeder-fri Udledning af neurale Crest stamceller fra menneskelige pluripotente stamceller
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Neuroscience
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JoVE Journal Neuroscience
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Feeder-fri Udledning af neurale Crest stamceller fra menneskelige pluripotente stamceller

DOI:
10.3791/51609-v

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10:33 min

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May 22, 2014

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, , ,
1Developmental Biology, Center for Stem Cell Biology,Sloan-Kettering Institute for Cancer Research, 2St. Giles Laboratory of Human Genetics of Infectious Diseases,The Rockefeller University

Chapters

  • 00:05Title
  • 01:38Plating of Human Pluripotent Stem Cells for Differentiation
  • 03:08Induction of Differentiation of Neural Crest Cells
  • 04:54Replating of Neural Crest Cells for Specification
  • 07:37Replating of FACS Sorted Cells
  • 08:56Results: Derivation of Neural Crest Cells from hPSCs using a Feeder Free Protocol
  • 10:09Conclusion

Summary

Automatic Translation

Automatic Translation

Neuralkam (NC) celler afledt af humane pluripotente stamceller (hPSC) har et stort potentiale til at modellere menneskelig udvikling og sygdom og for celleerstatningsterapier. Her et feeder-fri tilpasning af dag i vid udstrækning<em> In vitro</em> Differentiering protokol for afledning af NC celler fra hPSCs præsenteres.

Tags

Feeder-freeNeural Crest Progenitor CellsHuman Pluripotent Stem CellsIn Vitro DifferentiationEmbryonic DevelopmentPeripheral Nervous SystemMelanocytesMesenchymal CellsRegenerative MedicineDisease Modeling

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