Visualización de endocitosis mediada por clatrina de G receptores acoplados a proteínas en la Resolución Individual de eventos a través de TIRF de Microscopía
Clathrin-mediated endocytosis, a rapid and highly dynamic process internalizes many proteins, including signaling receptors. The protocol described here directly visualizes the kinetics of individual endocytic events. This is essential for understanding how core members of the endocytic machinery coordinate with each other, and how protein cargo influence this process.