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체외 합성에 인간의 세포에서 단백질 발현 유도의 mRNA를 수정의
JoVE Journal
Biology
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JoVE Journal Biology
In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells
DOI:

10:07 min

November 13, 2014

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Chapters

  • 00:05Title
  • 01:35Amplification of Plasmid Inserts and Addition of Poly T-tail by PCR
  • 02:48In Vitro Transcription
  • 03:59Treatment of Purified mRNA with Antartic Phosphatase
  • 05:17mRNA Transfection of Cells
  • 07:01Flow Cytometric Analyses of eGFP Expression in Cells
  • 08:14Results: Modified mRNA is Expressed in Human Cells
  • 09:34Conclusion

Summary

Automatic Translation

이 비디오 문서에서는, 우리는 세포에서 단백질 발현의 유도에 대한 수정의 mRNA의 체외 합성을 설명합니다.

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