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High Efficiency Differentiering af humane pluripotente stamceller til hjertemuskelceller og karakterisering ved hjælp af flowcytometri
JoVE Journal
Biology
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JoVE Journal Biology
High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
DOI:

13:13 min

September 23, 2014

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Chapters

  • 00:05Title
  • 01:43Passaging and Maintenance of Undifferentiated hPSCs in Monolayer Culture
  • 05:18Cardiomyocyte Induction of hPSCs by Selective Modulation of the Wnt Pathway
  • 08:22Fixation and Permeabilization of Cells for Intracellular Antigen Staining
  • 09:25Preparation of Cells for Flow Cytometry and Flow Cytometric Analysis
  • 10:34Characterization of Cardiomyocytes by Flow Cytometry and Electrophysiology
  • 12:26Conclusion

Summary

Automatic Translation

Artiklen beskriver den detaljerede metode til effektivt at differentiere humane pluripotente stamceller til kardiomyocytter ved selektivt at modulere Wnt vej, efterfulgt af flowcytometri analyse af referencemarkører at vurdere homogenitet og identitet af befolkningen.

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