Journal
/
/
Biofunctionalized Prussian Blue Nanopartikler til multimodal Molekylære billedbehandling
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
DOI:

11:28 min

April 28, 2015

, , , ,

Chapters

  • 00:05Title
  • 02:25Synthesis of Manganese Prussian Blue (MnPB) Nanoparticles
  • 04:09Biofunctionalization and Characterization of MnPB Nanoparticles
  • 05:29Fluorescent Labeling of Cells with Nanoparticles
  • 06:59Generating MRI Contrast on Cells with Nanoparticles
  • 09:01Results: Imaging of Cells Labeled with Prussian Blue Nanoparticles
  • 10:34Conclusion

Summary

Automatic Translation

This protocol describes the synthesis of biofunctionalized Prussian blue nanoparticles and their use as multimodal, molecular imaging agents. The nanoparticles have a core-shell design where gadolinium or manganese ions within the nanoparticle core generate MRI contrast. The biofunctional shell contains fluorophores for fluorescence imaging and targeting ligands for molecular targeting.

Related Videos

Read Article