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EAEモデルラットの中枢神経系から単核細胞の単離
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Biology
Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE
Please note that all translations are automatically generated.
Click here for the English version.
EAEモデルラットの中枢神経系から単核細胞の単離
DOI:
10.3791/527-v
•
20:51 min
•
December 04, 2007
•
Christine Beeton
,
K. George Chandy
1
Department of Physiology and Biophysics
,
University of California, Irvine (UCI)
Chapters
00:00
Title
00:10
Introduction
01:06
Cardiac Perfusion
05:36
Removing the Brain
09:11
Removing the Spinal Cord
11:18
Preparation of a Single-Cell Suspension
14:12
Percoll Gradient
17:41
Harvest of Mononuclear Cells
19:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このビデオでは、実験的自己免疫性脳脊髄炎を持つラットの中枢神経系から単核細胞を単離する方法を示します。
Tags
Isolation
Mononuclear Cells
Central Nervous System
Rats
EAE
Autoimmune Disease
Immune Cells
Infection
Poliomyelitis
Lyme Neuroborreliosis
Neurosyphilis
Video-protocol
Cardiac Perfusion
Saline Solution
Blood Contamination
Brain
Spinal Cord
Dilaceration
Single-cell Suspension
Percoll Gradient
Electrophysiology
Flow Cytometry (FACS)
Biochemistry
Sterile Conditions
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