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رد الفعل المحدد رصد الطيف الكتلي لالمطلق البروتين الكمي
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JoVE Journal Biology
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
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رد الفعل المحدد رصد الطيف الكتلي لالمطلق البروتين الكمي

العربية

Automatically Generated

16,836 Views

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09:04 min

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August 17, 2015

DOI:

10.3791/52959-v

DOI:
10.3791/52959-v

•

09:04 min
August 17, 2015

•

6 Views
, ,
1Cellular Networks Proteomics Unit, Laboratory of Systems Biology, National Institute of Allergy and Infectious Diseases,National Institutes of Health

Summary

Automatically generated

This protocol describes how to perform absolute quantification assays of target proteins within complex biological samples using selected reaction monitoring. It was used to accurately quantify proteins of the mouse macrophage chemotaxis signaling pathway. Target peptide selection, assay development, and qualitative and quantitative assays are described in detail.

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Cite this Article

Manes, N. P., Mann, J. M., Nita-Lazar, A. Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification. J. Vis. Exp. (102), e52959, doi:10.3791/52959 (2015).

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