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Mutlak Protein Niceleme Kütle Spektrometresi İzleme Seçilen Tepki
JoVE Journal
Biology
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JoVE Journal Biology
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Mutlak Protein Niceleme Kütle Spektrometresi İzleme Seçilen Tepki

16,834 Views

09:04 min

August 17, 2015

DOI:

09:04 min
August 17, 2015

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Summary

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This protocol describes how to perform absolute quantification assays of target proteins within complex biological samples using selected reaction monitoring. It was used to accurately quantify proteins of the mouse macrophage chemotaxis signaling pathway. Target peptide selection, assay development, and qualitative and quantitative assays are described in detail.

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