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Kupffer Cell Isolation for Nanoparticle Toxicity Testing
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Immunology and Infection
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JoVE Journal Immunology and Infection
Kupffer Cell Isolation for Nanoparticle Toxicity Testing

Kupffer Cell Isolation for Nanoparticle Toxicity Testing

DOI:
10.3791/52989-v

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09:49 min

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August 18, 2015

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, ,
1Institute of Pharmaceutical Science,King’s College London

Chapters

  • 00:05Title
  • 01:33Perfusion and Cell Collection
  • 04:03Kupffer Cell Purification
  • 06:18Modified Lactate Dehydrogenase Assay
  • 07:34Results: Nanoparticle Toxicity Testing on Purified Kupffer Cells
  • 09:11Conclusion

Summary

Automatic Translation

Automatic Translation

Liver macrophages, named Kupffer cells, are responsible for the capture of circulating nanoparticles. We describe here a method, of high cell purity and yield, for Kupffer cell isolation. The modified LDH assay is used here to measure the toxicity induced by carbon nanotubes in Kupffer cells.

Tags

Keywords: Kupffer CellsNanoparticle ToxicityIn Vitro AssaysPrimary CellsReticulo-endothelial SystemCell IsolationCollagenase DigestionDensity CentrifugationCarbon NanotubesLDH AssayCell Viability

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