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Imaging- и проточной цитометрии на основе анализа клеточной позиции и клеточного цикла в 3D меланомы сфероидов
JoVE Journal
Medicine
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JoVE Journal Medicine
Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids

Imaging- и проточной цитометрии на основе анализа клеточной позиции и клеточного цикла в 3D меланомы сфероидов

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10:44 min

December 28, 2015

DOI:

10:44 min
December 28, 2015

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Summary

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We describe two complementary methods using the fluorescence ubiquitination cell cycle indicator (FUCCI) and image analysis or flow cytometry to identify and isolate cells in the inner G1 arrested and outer proliferating regions of 3D spheroids.

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