A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:58Isolation of Nuclei
- 03:54Preparation of HeLa-S3 Nuclear Salt-extractable and Chromatin-bound Fractions
- 07:05Protein Complexes Purification
- 11:22Results: MyoD Interactors Identified by Tandem Affinity Purification Coupled to Mass Spectrometry
- 13:37Conclusion
MyoD is a myogenic transcription factor with a strong capacity to induce myogenic transdifferentiation of many fully differentiated non-muscle cell lines. The epigenetic mechanisms involved in this transdifferentiation are largely unknown. Here we describe a double-affinity purification method followed by mass spectrometry to exhaustively characterize MyoD partners.
