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JoVE Journal Medicine
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
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Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Article DOI: 10.3791/53928-v • 06:01 min • June 7th, 2016
June 7th, 2016 •
1, 2, 1
1Department of Biological Science, Center for Colon Cancer Research, University of South Carolina, 2Department of Pathology, Chonnam National University Medical School

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Here we present a protocol to detect miRNA expression in breast cancer patient samples using miRNA in situ hybridization.

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MiRNA Breast Cancer Tissues MiRNA In Situ Hybridization Tumor Suppressant Cellular Nuclearization Tissue Preparation Xylene Ethanol DEPC Treated Water Hydrophobic Barrier Pen 4% PFA PBS Wash Triton X-100 Proteinase K Solution Triethanolamine Acetic Anhydride
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