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JoVE Journal
Biology

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经过一蛋白饱和诱变图书馆的功能评价规范利用高通量测序
 
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经过一蛋白饱和诱变图书馆的功能评价规范利用高通量测序

Article DOI: 10.3791/54119-v 11:36 min July 3rd, 2016
July 3rd, 2016

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Summary

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Click here for the English version.

我们提出了一个协议为利用高通量测序蛋白的综合单站点饱和突变库功能评估。重要的是,这种方法采用正交引物对复图书馆建设和测序。提供了使用在氨苄青霉素的临床相关剂量选择的TE​​M-1β内酰胺酶代表性结果。

Tags

分子生物学,第113,诱变,饱和诱变,下一代测序,高通量测序,TEM-1β-内酰胺酶,抗生素抗性,正交引
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