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利用毛细管电泳从植物组织量化有机酸：一个测试用例检查<em>小粒种咖啡</em>种子
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Chemistry
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JoVE Journal Chemistry
Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
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利用毛细管电泳从植物组织量化有机酸：一个测试用例检查<em>小粒种咖啡</em>种子

DOI:
10.3791/54611-v

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10:13 min

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November 12, 2016

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, ,
1Center for Applied Plant Sciences,The Ohio State University, OARDC, 2Department of Plant Pathology,The Ohio State University, OARDC, 3Department of Horticulture and Crop Science,The Ohio State University, OARDC, 4OARDC Metabolite Analysis Cluster,The Ohio State University, OARDC

Chapters

  • 00:05Title
  • 00:49Sample Preparation
  • 02:30Organic Acid Standard Preparation
  • 04:34Organic Acid Extraction
  • 06:35SCCA Detection Run Execition and Data Collection
  • 08:18Results: Quantification of Organic Acid Levels in Green Coffee Beans
  • 09:35Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了检测和使用免费的纬向毛细管电泳从植物材料有机酸定量的方法。该方法的潜在应用中，确定在咖啡种子有机酸水平二次发酵的效果的一个例子中，提供了。

Tags

Capillary ElectrophoresisOrganic AcidsCoffea ArabicaCoffee SeedsShort Chain Carboxylic Acids (SCCAs)Sample PreparationLiquid Nitrogen GrindingAuthentic StandardsExternal And Internal StandardsCitric AcidMalic AcidAcetic AcidLactic AcidAdipic Acid

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