Administración sostenida de mitógenos de células β de los islotes intactos mouse<em> Ex Vivo</em> Uso Biodegradable poli (ácido láctico-co-glicólico) Microesferas
Here, we present methodology to generate and administer compound of interest-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres to intact mouse islets in culture with subsequent immunofluorescence analysis of β-cell proliferation. This method is suitable for determining the efficacy of candidate β-cell mitogens.