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JoVE Journal
Immunology and Infection

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SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
 
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SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

Article doi: 10.3791/54799
March 3rd, 2017

Summary March 3rd, 2017

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Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions.

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