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מתח ומות תא מפעיל באמצעות מיקרוסקופ Confocal הקונבנציונלי UV הליזר
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy
DOI:

10:18 min

February 03, 2017

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Chapters

  • 00:05Title
  • 01:21Prepare Zebrafish for Mounting and UV Cell Ablation
  • 04:18Confocal Microscope Set Up and Targeted Laser Ablation
  • 07:25Results: UV Laser Microscopy Induced Cell Stress and Death
  • 09:03Conclusion

Summary

Automatic Translation

Targeted manipulations to cause directed stress or death in individual cells have been relatively difficult to accomplish. Here, a single-cell-resolution ablation approach to selectively stress and kill individual cells in cell culture and living animals is described based on a standard confocal UV laser.

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