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JoVE Journal Biology
Metoder til at studere Epithelial Transport Protein Funktion og Expression i Native tarmen og Caco-2 celler dyrket i 3D
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Metoder til at studere Epithelial Transport Protein Funktion og Expression i Native tarmen og Caco-2 celler dyrket i 3D

Article DOI: 10.3791/55304-v • 11:27 min • March 16th, 2017
March 16th, 2017 •
*1, *1, 1, 1, 1, 1,2, 1,2, 1,2, 1
1Department of Medicine, University of Illinois at Chicago, 2Department of Research, Jesse Brown VA Medical Center
* These authors contributed equally

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Vi beskriver enkle metoder til at studere reguleringen af intestinal serotonin transporter (SERT) funktion og ekspression anvendelse af en in vitro cellekultur-model af Caco-2-celler dyrkes i 3D og en ex vivo model for muse tarmene. Disse metoder kan anvendes til studiet af andre epitel transportører.

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Cellular Biology transport funktion stripning slimhinde Ussing Afdeling 3D Caco-2 farvning af 3D celler serotonin transporteren SERT
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