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Journal
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Biology
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方法来研究上皮转运蛋白功能和表达在纯肠和Caco-2细胞生长在3D
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JoVE Journal
Biology
Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D
Please note that all translations are automatically generated.
Click here for the English version.
方法来研究上皮转运蛋白功能和表达在纯肠和Caco-2细胞生长在3D
DOI:
10.3791/55304-v
•
11:27 min
•
March 16, 2017
•
Arivarasu N. Anabazhagan*
1
,
Ishita Chatterjee*
1
,
Shubha Priyamvada
,
Anoop Kumar
,
Sangeeta Tyagi
,
Seema Saksena
2
,
Waddah A. Alrefai
2
,
Pradeep K. Dudeja
2
,
Ravinder K. Gill
1
Department of Medicine
,
University of Illinois at Chicago
,
2
Department of Research
,
Jesse Brown VA Medical Center
Chapters
00:05
Title
01:53
3D Caco-2 Cell Culture on a Gelatinous Protein Solution
03:23
Immunofluorescence Staining of Epithelial Transporters in 3D Caco-2 Cells
05:12
Measurement of 5-HT Uptake in Mouse Ileum
06:29
Equilibration and Tissue Treatment
07:41
Quantification of the Mucosal to serosal Flux (Jm-s) and
3
[H]-5-HT Accumulation
08:42
Results: The Expression and Function of SERT Protein Measured in Caco-2 3D Cell Cultures and Ileal Mucosa
Ex Vivo
09:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了简单的方法来研究肠血清素转运蛋白(SERT)的功能和表达的
使用体外
细胞培养的Caco-2细胞在三维生长模型和小鼠肠的
离体
模型的调节。这些方法适用于其他上皮转运的研究。
Tags
Epithelial Transport
Serotonin Transporter
SERT
Caco-2 Cells
3D Cell Culture
Ussing Chamber
Intestinal Epithelium
Electrolyte Transporters
Cell-cell Interactions
Cell-extracellular Matrix Interactions
Transport Function
Therapeutic Screening
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