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非人类灵长类动物脑组织的制备预嵌入免疫组化和电子显微镜
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
非人类灵长类动物脑组织的制备预嵌入免疫组化和电子显微镜
DOI:
10.3791/55397-v
•
11:55 min
•
April 03, 2017
•
Lara Eid
2
,
Martin Parent
1
Centre de recherche de l’Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience
,
Université Laval
,
2
Centre de recherche du CHU Sainte-Justine
Chapters
00:05
Title
00:43
Pre-embedding Immunohistochemistry
03:11
Osmification and Embedding for Electron Microscopy Observation
06:23
Sample Preparation for Ultrathin Sectioning and Observation Using a Transmission Electron Microscope
09:05
Results: Immunolabeling of the Squirrel Monkey Globus Pallidus for ChAT, SERT and TH
11:08
Conclusion
Summary
Automatic Translation
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在这里,我们提供了一种简单,低成本,和时间高效的协议进行化学固定与丙烯醛固定液灵长类脑组织,从而允许长期保存,其与预嵌入-免疫组化透射电子显微镜兼容。
Tags
Non-human Primate
Pre-embedding Immunohistochemistry
Electron Microscopy
Acrolein Fixation
Sodium Borohydride
Normal Serum
Cold Fish Gelatin
Primary Antibody
Secondary Antibody
Avidin-biotin-peroxidase
3
3′-Diaminobenzidine
Hydrogen Peroxide
Osmium Tetroxide
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