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Fabbricazione di Schiume matrice extracellulare-derivati e microcarriers come tessuto-specifica delle cellule, Cultura e piattaforme di distribuzione
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1Biomedical Engineering Graduate Program,The University of Western Ontario, 2Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry,The University of Western Ontario, 3Department of Chemical Engineering,Queen’s University, 4Department of Chemical & Biochemical Engineering, Faculty of Engineering,The University of Western Ontario
Chapters
- 00:05Title
- 00:52Decellularized Tissue Processing
- 01:41Extracellular Matrix (ECM) Suspension Preparation
- 04:01ECM-derived Foam Fabrication
- 04:58ECM-derived Microcarrier Fabrication via Electrospraying
- 07:11Preparation of Foams and Microcarriers for Cell Culture
- 08:55Results: Fabricated ECM-derived Foams and Microcarriers
- 10:36Conclusion
La matrice extracellulare tessuto-specifico (ECM) è un mediatore chiave della funzione delle cellule. Questo articolo descrive metodi per sintetizzare schiume e microcarriers che sono stabili in coltura senza la necessità di reticolazione chimica per applicazioni in 3D avanzata in colture di cellule in vitro o come bioscaffolds pro-rigenerativi ECM-derivato puri.
