/
/
Beoordeling van neuronale levensvatbaarheid met behulp van Fluorescein Diacetate-Propidium Iodide Dubbele Verf In Cerebellaire Granule Neuron Cultuur
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine,The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy,Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
Chapters
- 00:05Title
- 00:46Tissue Harvest from 8 Day Old Sprague-Dawley Rats
- 03:51FDA-PI Double Staining and FDA-PI-Hoechst Triple Staining in Cerebellar Granule Neuron Culture
- 05:30Assessment of Neuronal Viability
- 07:08Results: Double and Triple Stainings in CGN Culture
- 08:19Conclusion
Dit protocol beschrijft hoe om neuronale levensvatbaarheid nauwkeurig te meten met behulp van Fluorescein Diacetate (FDA) en Propidium Iodide (PI) dubbele kleuring in gekweekte cerebellar granule neuronen, een primaire neuronale cultuur die gebruikt wordt als een in vitro model in neurowetenschappen en neurofarmacologie onderzoek.
Tags
Neuronal ViabilityFluorescein Diacetate-propidium IodideCerebellar Granule Neuron CultureCell ViabilityNeuroscienceNeuropharmacologyGlial CellsNeuronsMISTA Cell CultureRat PupsCerebellumDissectionCentrifugationTrypsinDNaseSoybean Trypsin InhibitorMagnesium SulfateCalcium ChlorideCell Density6-well PlateCell Culture
