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Neuroscience

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JoVE Journal Neuroscience
Beurteilung der neuronalen Lebensfähigkeit unter Verwendung von Fluorescein-Diacetat-Propidium-Iodid-Doppelfärbung in Cerebellar-Granulat-Neuron-Kultur
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Beurteilung der neuronalen Lebensfähigkeit unter Verwendung von Fluorescein-Diacetat-Propidium-Iodid-Doppelfärbung in Cerebellar-Granulat-Neuron-Kultur

Article DOI: 10.3791/55442-v • 08:53 min • May 10th, 2017
May 10th, 2017 •
*1, *2, 1, 1, 1, 2, 3, 1, 2,4, 1,2
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
* These authors contributed equally

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Dieses Protokoll beschreibt die genaue Messung der neuronalen Lebensfähigkeit mit Fluorescein-Diacetat (FDA) und Propidium-Iodid (PI) Doppelfärbung in kultivierten Zerebellar-Granulat-Neuronen, einer primären neuronalen Kultur, die als in vitro- Modell in der Neurowissenschaften und der Neuropharmakologieforschung verwendet wird.

Tags

Neurowissenschaften Ausgabe 123 Cerebelläre Granulatneurone Fluoresceindiacetat Propidiumjodid Zelllebensfähigkeit Gliazellen Fluoreszenzmikroskopie
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