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Bedömning av neuronal livskraft med användning av fluoresceindiacetat-propidiumjodid dubbelfärgning i cerebellal granulär neurokultur
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1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine,The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy,Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
Chapters
- 00:05Title
- 00:46Tissue Harvest from 8 Day Old Sprague-Dawley Rats
- 03:51FDA-PI Double Staining and FDA-PI-Hoechst Triple Staining in Cerebellar Granule Neuron Culture
- 05:30Assessment of Neuronal Viability
- 07:08Results: Double and Triple Stainings in CGN Culture
- 08:19Conclusion
Detta protokoll beskriver hur man noggrant mäter neuronal lönsamhet med hjälp av fluoresceindiacetat (FDA) och propidiumjodid (PI) dubbelfärgning i odlade cerebellära granulärneuroner, en primär neuronkultur som används som in vitro- modell vid neurovetenskap och neurofarmakologiforskning.
Tags
Neuronal ViabilityFluorescein Diacetate-propidium IodideCerebellar Granule Neuron CultureCell ViabilityNeuroscienceNeuropharmacologyGlial CellsNeuronsMISTA Cell CultureRat PupsCerebellumDissectionCentrifugationTrypsinDNaseSoybean Trypsin InhibitorMagnesium SulfateCalcium ChlorideCell Density6-well PlateCell Culture
