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1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences,University of Antwerp, 2Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology,Ghent University, 3Department of Biochemistry , Radboud Institute for Molecular Life Sciences,Radboud University Medical Center
Chapters
- 00:05Title
- 00:47Seeding Cells in a 96-well Plate
- 01:48Setting Up the Microscope and Acquisition Protocol
- 03:10Loading of the Cells with CM-H2DCFDA and TMRM
- 04:18First and Second Live Imaging Rounds
- 06:22Image Processing and Analysis
- 09:35Results: Effect of Saquinavir on Primary Human Fibroblasts
- 10:38Conclusion
I dette papir præsenteres en mikroskopisk arbejdsstrøm med høj indhold til samtidig kvantificering af intracellulære ROS-niveauer samt mitokondrielt membranpotentiale og morfologi - i fællesskab betegnet mitokondriel morfofunktion - i levende adhærente celler ved anvendelse af de cellemeorante fluorescerende reportermolekyler 5- (og- 6) -chloromethyl-2 ', 7'-dichlorodihydrofluoresceindiacetat, acetylester (CM-H2 DCFDA) og tetramethylrhodaminmethylester (TMRM).
