Journal
/
/
脂滴隔离定量质谱分析
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
DOI:

10:23 min

April 17, 2017

, , ,

Chapters

  • 00:05Title
  • 00:37Preparation of Media for Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
  • 01:34SILAC-labeling and Amino Acid Incorporation Control
  • 03:15Lipid Droplet Isolation of SILAC-labeled Huh7.5 Cells
  • 07:59Analysis of Lipid Droplets Purity
  • 08:53Results: Qualitative and Quantitative Analysis of the Lipid Droplets
  • 09:57Conclusion

Summary

Automatic Translation

脂滴是几种病原体,包括丙型肝炎病毒(HCV)复制重要的细胞器。我们描述分离为相关蛋白的定量质谱脂滴的方法;它可以在各种条件下,如病毒感染,环境压力,或药物治疗中使用。

Related Videos

Read Article