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Journal / Biochemistry / Lipid Droplet Isolasjon for Quantitative…
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Biochemistry

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JoVE Journal Biochemistry
Lipid Droplet Isolasjon for Quantitative massespektrometri analyse
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Lipid Droplet Isolasjon for Quantitative massespektrometri analyse

Article DOI: 10.3791/55585-v • 10:23 min • April 17th, 2017
April 17th, 2017 •
1, 2, 2, 1
1Heinrich Pette Institute, Leibniz Institute for Experimental Virology, 2Core Facility Mass Spectrometric Proteomics, University Medical Center Hamburg-Eppendorf

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Lipiddråper er viktige organeller for replikasjon av flere patogener, inkludert hepatitt C-virus (HCV). Vi beskriver en metode for å isolere lipiddråper for kvantitativ massespektrometri av assosierte proteiner; den kan brukes under forskjellige forhold, for eksempel virus infeksjon, omgivende stress, eller medikamentell behandling.

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Biochemistry utgave 122 oljedråpe lipidlegemer kvantitativ massespektrometri stabil isotop merking med aminosyrer i cellekultur (SILAC) protein kvantifisering hepatitt C virus (HCV) virusinfeksjon
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