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Biochemistry
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脂滴隔离定量质谱分析
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Please note that all translations are automatically generated.
Click here for the English version.
脂滴隔离定量质谱分析
DOI:
10.3791/55585-v
•
10:23 min
•
April 17, 2017
•
Kathrin Rösch
,
Marcel Kwiatkowski
,
Hartmut Schlüter
,
Eva Herker
1
Heinrich Pette Institute
,
Leibniz Institute for Experimental Virology
,
2
Core Facility Mass Spectrometric Proteomics
,
University Medical Center Hamburg-Eppendorf
Chapters
00:05
Title
00:37
Preparation of Media for Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
01:34
SILAC-labeling and Amino Acid Incorporation Control
03:15
Lipid Droplet Isolation of SILAC-labeled Huh7.5 Cells
07:59
Analysis of Lipid Droplets Purity
08:53
Results: Qualitative and Quantitative Analysis of the Lipid Droplets
09:57
Conclusion
Summary
Automatic Translation
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Automatic Translation
脂滴是几种病原体,包括丙型肝炎病毒(HCV)复制重要的细胞器。我们描述分离为相关蛋白的定量质谱脂滴的方法;它可以在各种条件下,如病毒感染,环境压力,或药物治疗中使用。
Tags
Lipid Droplet Isolation
Mass Spectrometry Analysis
Quantitative Analysis
Hepatitis C Virus
SILAC Medium
Heavy And Light Labeled Cells
Protein Incorporation
SDS-PAGE
MS Analysis
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