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Genetics
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用荧光PCR毛细管电泳技术在基因型CRISPR / Cas9介导的敲除突变体的高通量形式
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Genetics
Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
Please note that all translations are automatically generated.
Click here for the English version.
用荧光PCR毛细管电泳技术在基因型CRISPR / Cas9介导的敲除突变体的高通量形式
DOI:
10.3791/55586-v
•
08:25 min
•
April 08, 2017
•
Muhammad Khairul Ramlee
,
Jing Wang
,
Alice M. S. Cheung
,
Shang Li
1
Cancer & Stem Cell Biology Programme
,
Duke-NUS Medical School
Chapters
00:05
Title
00:55
Sample Preparation and Amplification of CRISPER/Cas9 Regions with Fluorescent PCR
04:32
Capillary Gel Electrophoresis and Electropherogram Analysis
06:25
Results: Genotyping HEPG2 Targeted with CRISPER/Cas9 Construct Against NAP1L1
07:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里所描述的基因分型技术,荧光聚合酶链式反应(PCR),以毛细管凝胶电泳,其耦合,允许核酸酶介导敲除克隆的高通量基因分型。它规避所面临的其他基因分型技术的限制,并且比测序方法更具成本效益。
Tags
CRISPR/Cas9
Knockout Mutants
Genotyping
Fluorescent PCR
Capillary Gel Electrophoresis
High-throughput
Cell Culture
Cloning
Cell Lysis
PCR
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