/

/

时间依赖性增加对微电极阵列刺激神经元细胞培养物的网络响应

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

Please note that all translations are automatically generated. Click here for the English version.

时间依赖性增加对微电极阵列刺激神经元细胞培养物的网络响应

DOI:

10.3791/55726-v

•

10:45 min

•

May 29, 2017

•

Monica L. Gertz, Zachary Baker, Sharon Jose, Nathalia Peixoto

¹Krasnow Institute for Advanced Study,George Mason University, ²Neural Engineering, Bioengineering,George Mason University, ³Neural Engineering, Computer Science,George Mason University, ⁴Electrical and Computer Engineering,George Mason University

Chapters

00:05Title
01:04Embryo Removal and Brain Extraction
03:19Frontal Cortext Removal
04:46Cell Dissociation
06:48Plating Cells and Maintaining the Cultures
08:01Visual Inspections and Recording
09:24Results: Trained Networks Have Significantly Altered Spike Frequencies
10:19Conclusion

Summary

Automatic Translation

在多电极阵列上培养的小鼠神经元细胞在电刺激后显示出响应的增加。该协议演示了如何培养神经元，如何记录活动，以及如何建立协议来训练网络来应对刺激模式。

Tags

Keywords: Microelectrode Arrays Neuronal Cell Cultures Network Dynamics Learning And Memory Drug Toxicity Personalized Medicine Dissection Dissociation Laminin L-15 Medium

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

结合经颅磁刺激和功能磁共振成像检查默认模式网络

生成高度丰富的神经文化皮层组织块冷冻保存

器官型培养的神经元雪崩多电极阵列记录

谷氨酸和GABA快速微离子导入：一个有用的工具，调查突触整合

Sex Stratified Neuronal Cultures to Study Ischemic Cell Death Pathways

一个程序植入微丝的有组织阵列的单机录音清醒，举止动物

神经元共培养与单细胞精度的制备

接口3D工程神经细胞微电极阵列：一种创新<em>体外</em>实验模型

调查器官脊髓联合培养物中生长多电极阵列功能再生

采用组合运用各种方法，定义质膜交付在轴突分支和神经元形态发生中的作用

Read Article