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Modeling Neuronal død og Degeneration i musen primære cerebellare granulet neuroner
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Neuroscience
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JoVE Journal Neuroscience
Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

Modeling Neuronal død og Degeneration i musen primære cerebellare granulet neuroner

DOI:
10.3791/55871-v

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10:36 min

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November 06, 2017

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, , ,
1Lady Davis Institute for Medical Research,Jewish General Hospital, 2Integrated Program in Neuroscience,McGill University, 3Department of Cellular and Molecular Medicine,University of Ottawa, 4Department of Oncology, Faculty of Medicine,McGill University

Chapters

  • 00:05Title
  • 01:11Brain Dissection and Isolation of the Cerebellum
  • 03:11Mouse Cerebellar Granule Neuron Isolation and Culturing
  • 06:38Modeling Neuronal Injury
  • 08:09Results: Representative Images of Cerebellar Granule Neurons Infected with Different MOIs
  • 09:36Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en simpel metode til isolering og dyrkning af primære mus cerebral granulet neuroner (CGNs) fra 6-7 dage gamle hvalpe, effektiv transduktion af CGNs for tab og gevinst på funktion undersøgelser, og modellering NMDA-induceret neuronal excitotoxicity, lav-kalium-induceret celledød, DNA-skader og oxidativt stress ved hjælp af den samme kultur model.

Tags

Cerebellar Granule NeuronsNeuronal DeathNeurodegenerationPrimary Cell CultureExcitotoxicityOxidative StressDNA DamageDevelopmental EventsNeuronal ActivityMorphogenesisGrowth FactorsMouse Brain DissectionCerebellum IsolationMeningeal Removal

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