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Protocollo di immunoprecipitazione (ChIP) della cromatina per campioni embrionali bassa-abbondanza
JoVE Journal
Genetics
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JoVE Journal Genetics
Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
DOI:

12:47 min

August 29, 2017

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Chapters

  • 00:05Title
  • 00:57Preparation of Eggs and Microdissection of Chicken Spinal Neural Tube (SNT)
  • 04:12Crosslinking Proteins to DNA
  • 05:01Lysis, Sonication, and Immunoprecipitation
  • 06:33Washing, Elution, and Reversal of Crosslinking
  • 08:57Cellular Protein and RNA Digestion
  • 10:06Results: Evaluation of the ChIP Protocol by ChIP-seq Experiments
  • 11:48Conclusion

Summary

Automatic Translation

Qui, descriviamo un'immunoprecipitazione della cromatina (ChIP) e il protocollo di preparazione libreria ChIP-seq per generare profili epigenomic globale da campioni di embrionali di pollo di basso-abbondanza.

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